ISAAC: AN IMPROVED STRUCTURAL ANNOTATION OF ATTC AND AN INITIAL APPLICATION THEREOF

Abstract

<p>We introduce new software (ISAAC: Improved Structural Annotation of attC) to annotate cassette arrays in bacterial integrons by finding attI and attC sites, and to provide detailed annotation of the attC sites for analysis. We demonstrate an initial application of ISAAC by annotating the cassette complements of all the integrons we identified in the RefSeq bacterial genome database, and providing an analysis of the patterns of nucleotide frequencies at the structurally important positions in the attCs we’ve found.</p>