Suppressor-sensitive mutants of Bacteriophage PBS2

Abstract

<p>Suppressor-sensitive mutants of Bacillus subtilis phage PBS2 were isolated from chemically mutagenized lysates. These mutants were found to comprise thirty-eight distinct complementation groups. Mutants affected in DNA synthesis and host cell lysis were identified and characterized. Two complementation groups were found to be involved in the appearance of the phage-induced DNA polymerase activity. A linear genetic map was constructed based on the results of two and three-factor genetic crosses and linkage tests.</p>