Biochemical Investigations On An Asporogenous Mutant Of Bacillus Subtilis

Abstract

<p> Deletion in the chromosome of Bacillus subtilis strain Sp-H12-3 was demonstrated by Hg-Cs(2)SO(4) density gradient centrifugation. The base composition of the deleted DNA segments and transcription of m-RNA from these DNA segments were investigated. Physiological and biochemical studies of the mutant Sp-H12-3 yielded information on uridine derivatives which may be intimately associated with the process of sporulation. </p>