A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE

Abstract

Point-of-care DNA diagnostics for resource-limited settings require high sensitivity and low limits of detection but is constrained by a limitation on the complexity of instrumentation and resource consumption. To assist in the research and development of such technology, rapid-prototyping offers quick turnaround times from ideation to proof-of-concept testing at reduced costs. All-solution processed electrodes which exhibit micro/nano-scale wrinkling and porosity were rapidly-prototyped. Probe density was shown to be tunable with these electrodes and densities were greater than planar electrodes due to a surface area enhancement. Such electrodes also demonstrated favorable characteristics for the electrocatalytic detection of DNA hybridization. Characterization of hybridization efficiency for DNA biosensors often require the determination of probe and target DNA densities in separate experiments, relying on averaged measurements which lose device specificity. A new method to quantify hybridization efficiency was developed which allows the label-free, sequential determination of probe DNA and target DNA density in one experiment, allowing electrode-specific characterization of hybridization efficiency.