Studies on the Subcellular Distribution of Acid Phosphatase

Abstract

<p> Preliminary experiments indicated that lysosomes are present in rat liver and onion embryos. A differential centrifugation study was made of the intracellular distribution of acid phosphatase in pea embryo tissue in an attempt to show that this enzyme is enclosed by a membrane forming granules similar to the lysosomes of hepatic tissue. The results reveal that acid phosphatase is soluble under the conditions employed, but it is believed that this may well have resulted from excessive damage to the subcellular bodies during homogenization.</p>