Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/27611
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hossain, Zarin | - |
dc.date.accessioned | 2022-06-10T15:57:38Z | - |
dc.date.available | 2022-06-10T15:57:38Z | - |
dc.date.issued | 2022-02-18 | - |
dc.identifier.uri | http://hdl.handle.net/11375/27611 | - |
dc.description.abstract | Background: It’s been hypothesized that biophoton production is a result of the generation of excited species followed by relaxation of these excited species to a stable state. This can occur as a result of many stressors including irradiation from radioactive materials. Cell exposure to radium (Ra-226) has not been thoroughly explored for biophoton production. Furthermore, biophoton production is often associated with oxidative stress and cell death which is concurrently also an area of exploration. Objectives: The objective of this study was to characterize biophoton emission and additionally observe cell survival from α-irradiated HCT116+/+ cells via radium exposure. We aim to expand evidence that supports increased biophoton production and decreased cell survival as a result of oxidative stress and exposure to radioactive material. Methods: HCT116+/+ cells were standardly cultured in this study. For characterization of biophotons, cell groups were irradiated with 10, 100, 1000 and 10,000mBq/ml of Ra-226 for 24 hours. A photon counter was used to quantify counts. For characterization of cell survival, standard clonogenic assay techniques were used. Cell groups were irradiated with the same concentrations for Ra-226 and colonies were counted 7-9 days later. Results: No significant results were seen when observing biophoton counts from Ra-226 irradiated groups compared to a background count. Furthermore, there was a significant difference between Ra-226 irradiated groups when compared to a positive control known to produce biophotons. Some significant differences were seen in the surviving fraction of directly irradiated cells when looking at the different concentrations of Ra-226. However, overall there was a correlation that could be seen where an increase in concentration of Ra-226 resulted in a decreased surviving fraction of cells. Conclusions: The hypothesis of this study was partially supported where increased exposure and sensitivity to Ra-226 showed decreased cell survival, and assumed to have implications on biophoton production at higher concentrations although was not observed here at environmentally relevant concentrations. It was also concluded that biophoton production and oxidative stress are independent events. The significance of biophoton production as a radiation-induced bystander effect was explored as an implication of this study. | en_US |
dc.description.sponsorship | This work was supported by the Canadian Research Chairs Program [grant number 950- 221284]; National Chronic Fatigue and Immune Dysfunction Syndrome Foundation Inc.; Natural Sciences and Engineering Research Council Collaborative Research and Development Grant [grant number RGPIN293153-12]; CANDU Owners Group [grant number CRDPJ484381-15]; Bruce Power; and the Natural Sciences and Engineering Research Council of Canada Undergraduate Student Research Award program. Funding sources had no role in in study design; in the collection, analysis and interpretation of data; and in the writing of the report. | en_US |
dc.publisher | McMaster University | en_US |
dc.subject | Radiation | en_US |
dc.subject | Radiation Biology | en_US |
dc.subject | Radium | en_US |
dc.subject | Ra-226 | en_US |
dc.subject | Cell Culture | en_US |
dc.subject | Biophoton | en_US |
dc.subject | UVA | en_US |
dc.subject | Alpa-radiation | en_US |
dc.title | Characterization of UVA biophoton emission and survival of α-irradiated HCT116+/+ cells via radium exposure | en_US |
dc.type | Undergraduate thesis | en_US |
dc.contributor.department | Biology | en_US |
Appears in Collections: | Student Publications (Not Graduate Theses) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Hossain_Zarin_Final Sumission 2022 Feb_Biology & Pharmacology.pdf | 573.57 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License