Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/26543
Title: | Head-to-Head Accuracy Comparison of Three Commercial COVID-19 IgM/IgG Serology Rapid Tests |
Authors: | Andrey DO Cohen P Meyer B Torriani G Yerly S Mazza L Calame A Arm-Vernez I Guessous I Stringhini S Roux-Lombard P Fontao L Agoritsas T Stirnemann J Reny J-L Siegrist C-A Eckerle I Kaiser L Vuilleumier N |
Keywords: | COVID-19;ELISA;IgM/IgG serology;SARS-CoV-2;immunofluorescence;rapid test |
Publication Date: | 24-Jul-2020 |
Publisher: | MDPI AG |
Abstract: | <jats:p>Background: Comparative data of SARS-CoV-2 IgM/IgG serology rapid diagnostic tests (RDTs) is scarce. We thus performed a head-to-head comparison of three RDTs. Methods: In this unmatched case-control study, blood samples from 41 RT-PCR-confirmed COVID-19 cases and 50 negative controls were studied. The diagnostic accuracy of three commercially available COVID-19 RDTs: NTBIO (RDT-A), Orient-Gene (RDT-B), and MEDsan (RDT-C), against both a recombinant spike-expressing immunofluorescence assay (rIFA) and Euroimmun IgG ELISA, was assessed. RDT results concordant with the reference methods, and between whole blood and plasma, were established by the Kendall coefficient. Results: COVID-19 cases’ median time from RT-PCR to serology was 22 days (interquartile range (IQR) 13–31 days). Whole-blood IgG detection with RDT-A, -B, and -C showed 0.93, 0.83, and 0.98 concordance with rIFA. Against rIFA, RDT-A sensitivity (SN) was 92% (95% CI: 78–98) and specificity (SP) 100% (95% CI: 91–100), RDT-B showed 87% SN (95% CI: 72–95) and 98% SP (95% CI: 88–100), and RDT-C 100% SN (95% CI: 88–100) and 98% SP (95% CI: 88–100). Against ELISA, SN and SP were above 90% for all three RDTs. Conclusions: RDT-A and RDT-C displayed IgG detection SN and SP above 90% in whole blood. These RDTs could be considered in the absence of routine diagnostic serology facilities.</jats:p> |
metadata.dc.rights.license: | Attribution - CC BY |
URI: | http://hdl.handle.net/11375/26543 |
metadata.dc.identifier.doi: | https://doi.org/10.3390/jcm9082369 |
ISSN: | 2077-0383 2077-0383 |
Appears in Collections: | Faculty Publications (via McMaster Experts) |
Files in This Item:
File | Description | Size | Format | |
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Head-to-Head Accuracy Comparison of Three Commercial COVID-19 IgMIgG Serology Rapid Tests.pdf | Published version | 268.31 kB | Adobe PDF | View/Open |
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