Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/23143
Title: | Genotoxicity of 2-Nitrofluoranthene in Human Lymphoblastoid Cells In Vitro |
Authors: | Burgar-Suligoj, Tanya |
Advisor: | Tomkins, Darrell |
Department: | Biology |
Keywords: | gene;nitrofluoranthene;human;lymphoblastoid;in vitro |
Publication Date: | Jun-1996 |
Abstract: | A micronucleus assay was developed using HSC-3TO, a human continuous lymphoblastoid cell line (LCL) and cytochalasin B (Cyt-B) to block cytokinesis. Mitomycin C (MMC), a chemotherapeutic agent and an inducer of micronuclei in various human cell types, was used as a positive control. MMC induced micronuclei in HSC-3TO at levels over six times that of control cells when cells were treated with the Dā ā dose (i.e., the dose of MMC which was toxic to 50% of treated cells). It was found that harvest time was a very important variable and that a harvest time of 108 hours was optimal. 2-Nitrofluoranthene (2-NFA), a nitrated polycyclic aromatic hydrocarbon, the most abundant nitroarene found in the particulate organic matter fraction of ambient air and a potent direct-acting mutagen in the Ames Salmonella assay, was tested using the established CBMN assay protocol. Doses (20, 200 and 2000 ng/mL 2-NFA) were chosen based on the estimated yearly total body exposure and did not produce elevated levels of micronuclei at a dose of 100 times the estimated yearly total body exposure. |
URI: | http://hdl.handle.net/11375/23143 |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
burgar-suligoj_tanya_1996June_masters.pdf | 38.32 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.