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http://hdl.handle.net/11375/22744
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DC Field | Value | Language |
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dc.contributor.advisor | Brennan, John | - |
dc.contributor.author | Salvo, Elizabeth | - |
dc.date.accessioned | 2018-04-23T16:44:37Z | - |
dc.date.available | 2018-04-23T16:44:37Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | http://hdl.handle.net/11375/22744 | - |
dc.description.abstract | Microbial biosensor systems (MBS) are useful for analyte detection owing to their low cost, sensitivity, and selectivity for bioavailable analytes. Due to typically poor shelf-life and sensitivity to external conditions, there are few reports of MBS technology applied to simple analytical devices. The effectiveness of air-drying MBS in natural polymers was investigated as a novel preservation technique. Two colorimetric Escherichia coli MBS, a tetracycline-inducible reporter and an arsenate-inducible reporter, were dried on various substrates yielding novel MBS platforms. In proof-of-concept experiments performed in 96-well microplates, both systems demonstrated responsivity after air-drying in low concentrations of pullulan. However, the MBS were unresponsive following brief storage of 1 week. To improve the preservation of MBS, sensing strips were created by air-drying concentrated acacia gum-based MBS suspensions onto paper. Cells dried on these strips demonstrated responsivity upon solubilization in various tube-based assays. MBS sensing strip responsivity was demonstrated following storage for 6 weeks at 4 °C. Tetracycline-responsive sensing strips also performed well in assays using spiked lake water samples. Air-drying in natural polymers was an effective MBS preservation technique, and allowed for the creation of “mix and read” style assays which were simple, equipment-free and ready-to-use. | en_US |
dc.language.iso | en | en_US |
dc.subject | microbial biosensor | en_US |
dc.subject | paper-based | en_US |
dc.subject | air-drying | en_US |
dc.subject | natural polymers | en_US |
dc.subject | acacia gum | en_US |
dc.subject | pullulan | en_US |
dc.title | The air-drying of Escherichia coli reporters in natural polymers and incorporation into simple bioassays | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Chemical Biology | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MSc) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Salvo_Elizabeth_M_2017December_MSc.pdf | 1.28 MB | Adobe PDF | View/Open |
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