Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/22732
Title: | Consecutive spray drying to produce coated dry powder vaccines suitable for oral administration |
Authors: | Le Clair, Daniel Cranston, Emily Lichty, Brian Xing, Zhou Thompson, Michael |
Department: | Chemical Engineering |
Keywords: | vaccine;spray drying |
Publication Date: | 3-Apr-2018 |
Publisher: | ACS Publications |
Citation: | ACS Biomaterials Science and Engineering |
Abstract: | Current global vaccination programs are challenged by costs associated with vaccine cold chain storage and administration. A solid, thermally stable oral dosage form for vaccines would alleviate these costs but is difficult to produce due to general vaccine instability and the complication of bypassing the gastric barrier. We have developed a novel consecutive spray drying method that is suitable for use with biologics and employs Eudragit L100 polymer as the enteric coating. More specifically, in step 1, recombinant replication deficient human type-5 adenovirus and vesicular stomatitis virus were encapsulated by spray drying with sugars from a water solution, and in step 2 the microparticles from step 1 were suspended in ethanol with Eudragit and spray dried again. Up to 25% of the starting material was fully encapsulated within the enteric coating and encapsulation efficiency was largely dependent on spray gas flow rate and the solids concentration in the feed. After step 2, the coated vaccine-sugar particles maintained their thermostability and were slightly larger in size with a rugous surface morphology compared to the particles produced in step 1. The coated particles retained viral vector activity in vitro after 15 minute incubation in 1 M HCl (simulating the stomach environment) and anhydrous ethanol (to dissolve the Eudragit outer shell). The production of dry, orally administered vaccine particles from consecutive spray drying offers the potential to remedy a number of vaccine storage, transportation and administration limitations. |
URI: | http://hdl.handle.net/11375/22732 |
Identifier: | 10.1021/acsbiomaterials.8b00117 |
Appears in Collections: | Faculty Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
acs-manuscript-rev.docx | 3.96 MB | Microsoft Word XML | View/Open |
This item is licensed under a Creative Commons License