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http://hdl.handle.net/11375/22478
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DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | O'Donnell, M | - |
dc.contributor.author | Rheault, Mark | - |
dc.date.accessioned | 2018-01-09T18:57:31Z | - |
dc.date.available | 2018-01-09T18:57:31Z | - |
dc.date.issued | 1999-09 | - |
dc.identifier.uri | http://hdl.handle.net/11375/22478 | - |
dc.description.abstract | Malpighian (renal) tubules of the fruit fly Drosophila melanogaster consist of three functional domains: a non-secretory distal segment, a secretory main segment and a reabsorptive lower segment. In this study a computer-controlled micropositioning system and a self-referencing K^+ microelectrode were used to measure K^+ concentration gradients of extracellular unstirred layers associated with specific epithelial domains. K^+ fluxes were calculated from the measured gradients. This is the first time: that an accurate assessment of the concentration gradients of the unstirred layer of Drosophila melanogaster could be assessed due to the enhanced sensitivity of this self-referencing technique over conventional ion-selective microelectrodes. The technique permits high resolution spatial and temporal mapping of the flux patterns in response to stimulation or inhibition of ion transport. Variations in K^+ transport over time and at different sites suggest that transport is non-uniform within any one functional domain. | en_US |
dc.language.iso | en | en_US |
dc.subject | Drosophilia melanogaster | en_US |
dc.subject | epithelial K^+ | en_US |
dc.subject | spatial heterogeneity | en_US |
dc.subject | temporal heterogeneity | en_US |
dc.title | Automated Scanning Microelectrode Analysis of Epithelial K^+ Transport in Malpighian Tubules of Drosophilia melanogaster: Evidence for Spatial and Temporal Heterogeneity | en_US |
dc.title.alternative | Automated K^+ Scanning Microelectrode Analysis | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biology | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MSc) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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rheault_mark_r_Sept2009_masters.pdf | 13.56 MB | Adobe PDF | View/Open |
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