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http://hdl.handle.net/11375/22227
Title: | THE ROLE OF AMPK IN THE EXPRESSION OF THE DAPC |
Other Titles: | THE ROLE OF AMPK IN THE EXPRESSION OF THE DYSTROPHIN-ASSOCIATED PROTEIN COMPLEX IN SKELETAL MUSCLE |
Authors: | Dial, Athan |
Advisor: | Ljubicic, Vladimir |
Department: | Kinesiology |
Keywords: | AMP-activated protein kinase, dystrophin-associated protein complex, skeletal muscle, utrophin, peroxisome proliferator-activated receptor γ coactivator-1α |
Publication Date: | 2017 |
Abstract: | The dystrophin-associated protein complex (DAPC) provides a mechanical link between the intracellular cytoskeleton and extracellular matrix, serving as a mechanosensor and signal transducer across the sarcolemma. Pharmacological stimulation of AMP-activated protein kinase (AMPK) induces the expression of DAPC components in skeletal muscle, whereas physiological reductions in AMPK are associated with DAPC dysfunction. We sought to determine whether AMPK was necessary for the maintenance of DAPC expression in skeletal muscle. Fast glycolytic extensor digitorum longus (EDL) and slow oxidative soleus (SOL) muscles from wild-type (WT) mice, as well as from littermates deficient in both isoforms of the AMPK-β subunit in skeletal muscle (MKO) were analyzed. DAPC mRNA levels, as well as protein expression and localization were similar between genotypes, with the exception of nNOS, which displayed a compensatory sarcolemmal enrichment in MKO muscles. The content of transcriptional and post-transcriptional regulators of the DAPC, such as PGC-1α and KSRP, were also not affected by the loss of AMPK. However, MyoD and myogenin expression was significantly diminished in MKO muscles, which is consistent with previous reports of myopathy in these animals. Furthermore, we observed decrements in extrasynaptic utrophin expression selectively in MKO SOL muscles, despite an adaptive accumulation of PGC-1α at the sarcolemmal compartment. Collectively the evidence indicates that AMPK is sufficient, but not essential for the maintenance of DAPC expression in skeletal muscle. However, AMPK is required for preserving extrasynaptic utrophin levels in slow, oxidative muscles, which underscores the role of AMPK in the gene expression of this disease modifying protein. |
URI: | http://hdl.handle.net/11375/22227 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Dial_Athan_G_FinalSubmissionAugust2017_MSc.pdf | 2.21 MB | Adobe PDF | View/Open |
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