Temperature-Sensitive Mutants of Bacteriophage PBS 2

Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/17968

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DC Field	Value	Language
dc.contributor.advisor	Takahashi, I.	-
dc.contributor.author	Herrington, Muriel Bella	-
dc.date.accessioned	2015-08-26T18:17:23Z	-
dc.date.available	2015-08-26T18:17:23Z	-
dc.date.issued	1973-11	-
dc.identifier.uri	http://hdl.handle.net/11375/17968	-
dc.description.abstract	<p> Temperature-sensitive mutants of the bacteriophage PBS 2 were isolated from lysates treated with various mutagens. Complementation tests assigned the mutants to 10 cistrons. The mutants were mapped by two factor crosses and formed a linear map approximately 50 recombination percent in length. </p> <p> A method for phage transformation was developed. By the use of wild type DNA fragments fractionated according to their guanine + cytosine content in HgCs₂SO₄ gradients, it was possible to determine the base composition of certain regions of the chromosomes. </p>	en_US
dc.language.iso	en	en_US
dc.subject	biology	en_US
dc.subject	temperature-sensitive mutant	en_US
dc.subject	bacteriophage; PBS 2	en_US
dc.title	Temperature-Sensitive Mutants of Bacteriophage PBS 2	en_US
dc.contributor.department	Biology	en_US
dc.description.degreetype	Thesis	en_US
dc.description.degree	Doctor of Philosophy (PhD)	en_US
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