Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/17744
Title: | The Biogenesis of Mitochondria in Mammalian Cells (L Cells) |
Authors: | Fettes, Ivy Marlys |
Advisor: | Freeman, K. B. |
Department: | Biochemistry |
Keywords: | biochemistry, biophysics, molecular biology;biogenesis;mitochondria;mammalian cell;L cell |
Publication Date: | Aug-1970 |
Abstract: | Chloramphenicol has been used to study mitochondrial biogenesis in mammalian cells by examining its effect on: the incorporation of radioactive amino acids into protein by isolated mitochondria, the growth of L cells, the level of representative enzymes and cytochromes in the mitochondria and cytoplasm and the structure of mitochondria and L cells. A reversible inhibition of synthesis of cytochrome c oxidase was obtained by treating cells with D-threo-chloramphenicol for 90 hr. Recovery of cytochrome c oxidase activity was inhibited by cycloheximide, an inhibitor of cytoplasmic protein synthesis. Cycloheximide also reversibly inhibited cytochrome c oxidase formation in cells which were not treated with D-chloramphenicol. It is suggested that the mitochondria and the nucleus have a joint control in the formation of a functionally active cytochrome c oxidase enzyme. |
URI: | http://hdl.handle.net/11375/17744 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Fettes_Ivy_M_1970Aug_PhD.pdf | 26.92 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.