Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/16437
Title: | Rapid Prototyping Of Wrinkled Nano-/Micro-Structured Electrodes For Electrochemical DNA Detection |
Authors: | Woo, Stephen Minju |
Advisor: | Soleymani, Leyla |
Department: | Biomedical Engineering |
Keywords: | Biosensors;Electrochemistry;Biomedical Engineering;Rapid Prototyping;Point-of-care Testing;Molecular Diagnostics;Lab-On-A-Chip |
Publication Date: | Nov-2014 |
Abstract: | Rapid, point-of-care infectious disease diagnostics have the potential to dramatically improve health care provision in low-income world regions. However, the development of technologies such as electrochemical DNA biosensors is hindered by slow turnaround times from design to working prototype. In order to facilitate biosensor development, a rapid prototyping method has been applied to the fabrication of wrinkled nano-/micro-structured electrodes in this work. An electrocatalytic DNA hybridization detection scheme is optimized for use with the wrinkled electrodes by adjusting the concentrations of redox agents FiCN and RuHex. Characterization of the electrodes by electrochemical and fluorescence-based methods showed tunability of important detection-related parameters – namely, the density of DNA probe molecules and the hybridization-induced electrocatalytic signal change – by altering parameters of deposition time, molar fraction of DNA probes relative to diluent molecules, and thickness of the wrinkled gold film. |
URI: | http://hdl.handle.net/11375/16437 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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SW Thesis Final-For submission.docx | Complete MASc Thesis | 8.96 MB | Microsoft Word XML | View/Open |
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