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http://hdl.handle.net/11375/11597
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DC Field | Value | Language |
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dc.contributor.advisor | Inman, Mark D | en_US |
dc.contributor.advisor | Janssen, Luke | en_US |
dc.contributor.advisor | Jordana, Manel | en_US |
dc.contributor.author | Dalle, Ave J Christopher | en_US |
dc.date.accessioned | 2014-06-18T16:55:30Z | - |
dc.date.available | 2014-06-18T16:55:30Z | - |
dc.date.created | 2011-11-11 | en_US |
dc.date.issued | 2012-04 | en_US |
dc.identifier.other | opendissertations/6554 | en_US |
dc.identifier.other | 7523 | en_US |
dc.identifier.other | 2345943 | en_US |
dc.identifier.uri | http://hdl.handle.net/11375/11597 | - |
dc.description.abstract | <p><strong>Introduction</strong></p> <p>The airway epithelium, which acts as a protective barrier, is impaired in asthmatic patients and may contribute to abnormal airway function. Chronic inflammation, a feature of asthma, is associated with structural changes in the airway epithelium including the transformation of columnar epithelial cells into mucin secreting goblet cells. Human epithelial cells exposed to Interleukin-13 (IL-13) <em>in vitro</em> resulted in goblet cell metaplasia and a significant drop in transepithelial resistance, indicating that barrier function is impaired.</p> <p><strong>Aim</strong></p> <p>We sought to determine whether goblet cell metaplasia alone is sufficient to impair airway epithelial barrier function <em>in vivo</em>.</p> <p><strong>Methods</strong></p> <p>Female BALB/c mice were infected with an adenovirus to overexpress IL-13, a control adenovirus, or no virus. Barrier integrity was assessed via single-photon emission computed tomography (SPECT) imaging by measuring the dispersion of technetium-labeled diethylene triamine pentaacetic acid (<sup>99m</sup>Tc-DTPA) out of the lungs over time. Lung sections were stained by Periodic acid-Schiff to detect the presence of mucin-containing goblet cells.</p> <p><strong>Results</strong></p> <p>IL-13 exposure resulted in goblet cell metaplasia and associated airway hyperresponsiveness to methacholine. However, there was no significant increase in dispersion of <sup>99m</sup>Tc-DTPA over time from the airways in IL-13 overexpressed mice compared to control mice.</p> <p><strong>Conclusion</strong></p> <p>IL-13 induced goblet cell metaplasia did not impair the airway epithelial barrier to <sup>99m</sup>Tc-DTPA in our <em>in vivo</em> mouse model. Therefore, we conclude that epithelial dysfunction to DTPA observed in human asthmatics and in animal models of asthma are not due to IL-13 induced goblet cell metaplasia.</p> | en_US |
dc.subject | epithelial barrier | en_US |
dc.subject | IL-13 | en_US |
dc.subject | asthma | en_US |
dc.subject | Biological Phenomena, Cell Phenomena, and Immunity | en_US |
dc.subject | Circulatory and Respiratory Physiology | en_US |
dc.subject | Biological Phenomena, Cell Phenomena, and Immunity | en_US |
dc.title | The Effect of Goblet Cell Metaplasia On Airway Barrier Integrity | en_US |
dc.type | thesis | en_US |
dc.contributor.department | Medical Sciences (Division of Physiology/Pharmacology) | en_US |
dc.description.degree | Master of Science (MSc) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
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fulltext.pdf | 887.77 kB | Adobe PDF | View/Open |
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