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http://hdl.handle.net/11375/9507
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DC Field | Value | Language |
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dc.contributor.advisor | Valliant, John F. | en_US |
dc.contributor.author | Hicks, Justin W. | en_US |
dc.date.accessioned | 2014-06-18T16:47:23Z | - |
dc.date.available | 2014-06-18T16:47:23Z | - |
dc.date.created | 2011-06-07 | en_US |
dc.date.issued | 2010 | en_US |
dc.identifier.other | opendissertations/4624 | en_US |
dc.identifier.other | 5642 | en_US |
dc.identifier.other | 2050678 | en_US |
dc.identifier.uri | http://hdl.handle.net/11375/9507 | - |
dc.description.abstract | <p>To produce Tc-based radiopharmaceuticals in high effective specific activity the excess precursor ligand must be removed. A new and convenient solid-phase ligand capture method of a resin-bound Cu chelate was recently developed to remove unlabeled ligand.[1] Herein, the impact ofsize on the purification was evaluated using three welldefined resin sizes and a model chelate system, where the average capture efficiency was 89.1 ± 3.3% and loss due to non-specific binding was 23.4 ± 1.6%.</p> <p>A complementary solution-phase strategy was developed employing fluorous chemistry to remove unlabeled chelate-derivatives with less loss of product. The novel fluorous analog to the solid-phase copper chelate was synthesized in three steps (70% overall yield). Using the same model Tc(I) chelate, >99% ofthe ligand was captured by the fluorous ligand capture (FLC) method and the extent of non-specific binding was 65% lower than the solid-phase equivalent. A peptide conjugate was then used to further test the general utility ofthe FLC method with comparable ligand removal (95.1 ±0.8%) and non-specific binding (12.1 ± 1.2%).</p> <p>Attempts were made to automate the FLC method using a microfluidic device with no detrimental effect to either the radiochemical yield (4.15 ± 1.0% non-specific binding) or the efficiency of ligand capture (99.74 ± 0.04%). A second strategy involved preloading the FLC agent onto fluorous silica and capturing the excess ligand (99.85 ± 0.02%) and peptide (95± 1%) as the radiolabeling mixture was washed over the cartridge with 2.25 ± 0.55% and 33.3 ± 3.2% product lost, respectively. This approach can now be incorporated into a new generation of instant kits or an integrated microfluidic preparation of molecular imaging probes in high effective specific activity.</p> | en_US |
dc.subject | Chemistry | en_US |
dc.subject | Chemistry | en_US |
dc.title | Fluorous-Phase Purification of ⁹⁹ᵐTc Radiopharmaceuticals | en_US |
dc.type | thesis | en_US |
dc.contributor.department | Chemistry | en_US |
dc.description.degree | Master of Science (MS) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Size | Format | |
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fulltext.pdf | 3.46 MB | Adobe PDF | View/Open |
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