Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/9221
Title: | Microfluidic Reference Electrode for Use in BioFET Sensor Systems |
Authors: | SafariMohsenabad, Salman |
Advisor: | Selvaganapathy, P. R. Deen, M. J. |
Department: | Mechanical Engineering |
Keywords: | Mechanical Engineering;Mechanical Engineering |
Publication Date: | Nov-2010 |
Abstract: | <p>Biosensors, used in medical diagnostics, increasingly use genomic information (DNA) to rapidly and accurately determine the species present in the sample. Since, the DNA inherently has a negative charge, electrical methods provide a direct technique to sense it. This transduction has been achieved by using a biological Field-Effect-Transistor (BioFET) structure, where hybridization of a single stranded DNA (indicative of the biological species) with a complementary strand from the sample solution causes a change in the transistor characteristics that could be read out electrically. The accuracy of sensing using the BioFET is critically dependent on imposition of a highly stable potential which is performed using a reference electrode.</p> <p>Design and fabrication of a miniaturized silver/silver chloride (Ag/AgCI) reference electrode is introduced in this thesis for use in BioFET. The electrode consists of Ag/AgCl wire which is embedded into a PDMS microchannel enclosed by a microcontact printed nanoporous polycarbonate membrane. The microchannel is filled with KCI solution as the internal solution.</p> <p>By modifying the electrodeposition method, nanosheet AgCI structure was grown rather than the conventional globular morphology. The bare Ag/AgCl potential drift with the former morphology was found to be</p> <p>In conclusion, a modified electrodeposition method and free-diffusion liquid junction micro fluidic reference electrode is proposed to improve the stability and extend the lifetime of a reference electrode with very low potential drift.</p> |
URI: | http://hdl.handle.net/11375/9221 |
Identifier: | opendissertations/4363 5381 2041352 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Size | Format | |
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fulltext.pdf | 3.96 MB | Adobe PDF | View/Open |
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