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http://hdl.handle.net/11375/9110
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DC Field | Value | Language |
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dc.contributor.advisor | Ghosh, R. | en_US |
dc.contributor.author | Mah, Kor-Zheng | en_US |
dc.date.accessioned | 2014-06-18T16:45:39Z | - |
dc.date.available | 2014-06-18T16:45:39Z | - |
dc.date.created | 2011-05-27 | en_US |
dc.date.issued | 2009 | en_US |
dc.identifier.other | opendissertations/4262 | en_US |
dc.identifier.other | 5281 | en_US |
dc.identifier.other | 2035594 | en_US |
dc.identifier.uri | http://hdl.handle.net/11375/9110 | - |
dc.description.abstract | <p>Environment-responsive paper-supported composite membranes, consisting of interpenetrating network of poly(<em>N</em>-vinyl caprolactam) and cellulose fibres, suitable for hydrophobic interaction membrane chromatography of proteins were prepared. These membranes could be made to switch between hydrophobic and hydrophilic states in a reversible manner by addition or removal of anti-chaotropic salts. A series of systematic studies were conducted to examine the effects of polymer loading and salt concentration on hydraulic and antibody binding properties of these membranes. A membrane possessing a satisfactory balance of hydraulic permeability and antibody binding was further examined for use in an immunoglobulin G (IgG) purification process. The membrane demonstrated low fouling tendency, satisfactory IgG binding (c.a. 12 mg/ml bed volume), and was successfully used for IgG purification from simulated cell culture supernatant. The results obtained demonstrate that the poly(<em>N</em>-vinyl caprolactam)-paper composite and indeed similar material could be used as inexpensive membranes adsorbers for protein purification.</p> | en_US |
dc.subject | Chemical Engineering | en_US |
dc.subject | Chemical Engineering | en_US |
dc.title | Environment-Responsive Paper-supported Membrane for Chromatographic Bio-separations: Preparation, Characterization and Application | en_US |
dc.type | thesis | en_US |
dc.contributor.department | Chemical Engineering | en_US |
dc.description.degree | Master of Applied Science (MASc) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Size | Format | |
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fulltext.pdf | 2.71 MB | Adobe PDF | View/Open |
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