Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/9110
Title: | Environment-Responsive Paper-supported Membrane for Chromatographic Bio-separations: Preparation, Characterization and Application |
Authors: | Mah, Kor-Zheng |
Advisor: | Ghosh, R. |
Department: | Chemical Engineering |
Keywords: | Chemical Engineering;Chemical Engineering |
Publication Date: | 2009 |
Abstract: | <p>Environment-responsive paper-supported composite membranes, consisting of interpenetrating network of poly(<em>N</em>-vinyl caprolactam) and cellulose fibres, suitable for hydrophobic interaction membrane chromatography of proteins were prepared. These membranes could be made to switch between hydrophobic and hydrophilic states in a reversible manner by addition or removal of anti-chaotropic salts. A series of systematic studies were conducted to examine the effects of polymer loading and salt concentration on hydraulic and antibody binding properties of these membranes. A membrane possessing a satisfactory balance of hydraulic permeability and antibody binding was further examined for use in an immunoglobulin G (IgG) purification process. The membrane demonstrated low fouling tendency, satisfactory IgG binding (c.a. 12 mg/ml bed volume), and was successfully used for IgG purification from simulated cell culture supernatant. The results obtained demonstrate that the poly(<em>N</em>-vinyl caprolactam)-paper composite and indeed similar material could be used as inexpensive membranes adsorbers for protein purification.</p> |
URI: | http://hdl.handle.net/11375/9110 |
Identifier: | opendissertations/4262 5281 2035594 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Size | Format | |
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fulltext.pdf | 2.71 MB | Adobe PDF | View/Open |
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