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http://hdl.handle.net/11375/8958
Title: | Development of Paper-based Devices for Diagnostics and Biosensing |
Authors: | Leung, Vincent |
Advisor: | Filipe, Carlos Pelton, Robert Dr. Todd Hoare and Dr. Emily Cranston |
Department: | Chemical Engineering |
Keywords: | Paper-based devices;microfluidics;biosensors;diagnostics;Other Chemical Engineering;Other Chemical Engineering |
Publication Date: | Oct-2011 |
Abstract: | <p>Research in paper-based analytical devices has been increasing rapidly in recent years. Manyof these devices are used as low-cost alternatives for diagnostics and biosensing. In this work,two novel paper-based technologies were developed.</p> <p>The first paper-based technology achieved was measuring streaming potential on paper-based microfluidic devices. The streaming potential measurements were able to detect the presence of adsorbed polyvinylamine or potassium polyvinylsulfate in paper-based microfluidic channels.</p> <p>The measured streaming potential ranged from -80 mV to 80 mV and the polarity was sensitive to the adsorbed polymer. Furthermore, the measured streaming potential on paper treated with BSA showed a polarity switch when the pH was changed from below the pKa to above the pKa of BSA. Lastly, streaming potential measurements may provide an electronic interface for paperbased sensors.</p> <p>The second technology developed was a paper-based chromatographic pre-concentration device for biological and chemical applications. The device successfully concentrated a protein, streptavidin, via biotinylated microgels immobilized onto a selected area of the filter paper. The device was able to process a large volume of fluid with the incorporation of a passive pump made of superabsorbent polymer. The concentration factor achieved by the device was over 3000-fold. The flow dynamics through the paper was modeled using Darcy’s law. This technology could be an excellent low-cost alternative for biochemical analysis for samples thatrequire preconcentration, especially for the analysis of trace compounds in wastewater and drinking water.</p> |
URI: | http://hdl.handle.net/11375/8958 |
Identifier: | opendissertations/4123 5123 2014937 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
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fulltext.pdf | 3.48 MB | Adobe PDF | View/Open |
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