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http://hdl.handle.net/11375/8765
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DC Field | Value | Language |
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dc.contributor.advisor | Diamond, J. | en_US |
dc.contributor.author | Lourenssen, Sandra | en_US |
dc.date.accessioned | 2014-06-18T16:43:54Z | - |
dc.date.available | 2014-06-18T16:43:54Z | - |
dc.date.created | 2011-03-05 | en_US |
dc.date.issued | 1997 | en_US |
dc.identifier.other | opendissertations/3942 | en_US |
dc.identifier.other | 4959 | en_US |
dc.identifier.other | 1851881 | en_US |
dc.identifier.uri | http://hdl.handle.net/11375/8765 | - |
dc.description.abstract | <p>This thesis involved the use of mutant and transgenic mice to examine the role of<br />various factors in reparative growth of the adult peripheral nervous system. These animals<br />have genetic defects that could be anticipated to affect reparative growth of sensory nerves,<br />and included mice that are transgenic for the heavy chain neurofilament (NFH)lacZ fusion<br />construct, mice mutant either in the steel or the c-kit locus, and mice with a null mutation in<br />the gene coding for the low-affinity neurotrophin receptor (p75^NGFR).</p> <p>Both collateral sprouting and regeneration occurred in the NFHlacZ mice at a rate<br />similar to that observed in control mice. Therefore, overexpression of the NFHlacZ construct<br />had no effect on reparative growth of sensory nerves.</p> <p>Interestingly, regeneration of all three fibre types examined was impaired in mice<br />that have a mutation in the gene coding for Steel or c-Kit, and occured at a rate of 1/2 to 2/3<br />of that observed in control animals. However, the rate ofcollateral sprouting was normal.</p> <p>Although the sensory nerves of p75^NGFR mice underwent normal regeneration,<br />collateral sprouting was completely absent. Interestingly, exogenous NGF induced this form<br />of growth in these animals.</p> <p>In addition, there was a significant decrease in the number of axons comprising all three fibre types in the sensory nerves in both the SI/Sld, WIW and p75^NGFR mice,<br />suggesting that the molecules involved also play a role in the development of sensory<br />neurons.</p> <p>NGF-induced hyperalgesia was not evident in the control or knockout animals upon<br />exogenous administration of either 1 or 2.5μg/g of NGF. However, administration of<br />2.5μg/g of NGF resulted in a significant mechanical hyperalgesia in both the control and<br />knockout mice. Thus, it appears that the low-affinity neurotrophin receptor does not play a<br />role in NGF-induced hyperalgesia.</p> | en_US |
dc.title | Mechanisms of reparative growth in adult peripheral cutaneous nerves | en_US |
dc.type | thesis | en_US |
dc.contributor.department | Biomedical Sciences | en_US |
dc.description.degree | Doctor of Philosophy (PhD) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
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fulltext.pdf | 4.72 MB | Adobe PDF | View/Open |
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