Skip navigation
  • Home
  • Browse
    • Communities
      & Collections
    • Browse Items by:
    • Publication Date
    • Author
    • Title
    • Subject
    • Department
  • Sign on to:
    • My MacSphere
    • Receive email
      updates
    • Edit Profile


McMaster University Home Page
  1. MacSphere
  2. Open Access Dissertations and Theses Community
  3. Open Access Dissertations and Theses
Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/7899
Title: The fidelity of in vitro protein synthesis and its implications for the aging of human cells
Authors: Wojtyk, Ivan Roman
Advisor: Goldstein, Samuel
Department: Medical Sciences
Keywords: Medical Sciences;Medical Sciences
Publication Date: Apr-1981
Abstract: <p>To test the error catastrophe theory of cell senescence, we have developed a cell-free protein synthetic system from human diploid fibroblasts capable of translating both the synthetic mRNA, poly (u), and endogenous cellular mRNA in vitro. We have measured the fidelity of poly(U)-directed protein synthesis in extracts from a variety of cells. The results contradict the error catastrophe in several ways: - cells from subjects suffering maladies of premature aging, progeria and Werner Syndrome, exhibited error frequencies within the normal range. - cells from an old donor did not have elevated error frequency of protein synthesis. - early-passage (young) normal cells had error frequencies higher than those of late-passage (old) cells. The error frequency in one normal strain of fibroblasts declined throughout its tissue culture lifespan. Cells maintained in the post-mitotic (terminal) state, in which there was no cell selection, exhibited a constant error frequency over 16 weeks. In a series of experiments using clonal populations of cells, it became evident that the decline in error frequency as a function of passage was dependent on the clonal heterogeneity of the mass culture. In no experiment, however, could any correlation between the error frequency of protein synthesis and cellular growth parameters be made. Overall, the senescence characteristic of human diploid fibroblasts was independent of the measured fidelity of protein synthesis in vitro.</p>
URI: http://hdl.handle.net/11375/7899
Identifier: opendissertations/3146
4162
1452815
Appears in Collections:Open Access Dissertations and Theses

Files in This Item:
File SizeFormat 
fulltext.pdf
Open Access
7.67 MBAdobe PDFView/Open
Show full item record Statistics


Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.

Sherman Centre for Digital Scholarship     McMaster University Libraries
©2022 McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L8 | 905-525-9140 | Contact Us | Terms of Use & Privacy Policy | Feedback

Report Accessibility Issue