Skip navigation
  • Home
  • Browse
    • Communities
      & Collections
    • Browse Items by:
    • Publication Date
    • Author
    • Title
    • Subject
    • Department
  • Sign on to:
    • My MacSphere
    • Receive email
      updates
    • Edit Profile


McMaster University Home Page
  1. MacSphere
  2. Open Access Dissertations and Theses Community
  3. Open Access Dissertations and Theses
Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/7582
Title: Production and Characterization of Monoclonal Antibodies Against Herpes Simplex Virus
Authors: Newhook, Lawrence Andrew George
Advisor: Bacchetti, Silvia
Department: Medical Sciences
Keywords: Medical Sciences;Medical Sciences
Publication Date: Jul-1980
Abstract: <p>Methods for the production of hybrid cell lines (hybridomas) secreting monoclonal antibodies against specific antigenic determinants have recently been developed (Kohler G. and Milstein C., Nature 256, 495, 1975). Monospecific antibodies to Herpes simplex virus (HSV) antigens would greatly facilitate the analysis of HSV type-specific determinants, be of use in molecular studies of virus-cell interactions and could have potential applications in immunization against HSV infections. To this end, spleen cells from HSV2-immunized BALB/c mice have been fused to a BALB/c derived HGPRT myeloma line (Sp 2/0 Ag-14) and the resulting hybrids selected in HAT medium. From 13 successful fusions, 102 hybrid populations secreting antibodies recognizing antigenic determinants specified on HSV2-infected cells have been identified by FITC-immunofluorescence, an ELISA method or a ¹²⁵I-protein A binding assay. Ten of the positive hybrids have been cloned by limited dilutions to generate 124 monoclonal lines reacting specifically with HSV-infected but not with mock-infected cells. High titres of anti-HSV specific antibodies have also been detected in two ascitic fluids recovered from tumors induced in mice by injection of positive hybridomas. Preliminary data on the characterization of two hybridomas in terms of the subclass of immunoglobulins they secrete and the specificities of antibody they define has also been obtained.</p>
URI: http://hdl.handle.net/11375/7582
Identifier: opendissertations/2850
3852
1412354
Appears in Collections:Open Access Dissertations and Theses

Files in This Item:
File SizeFormat 
fulltext.pdf
Open Access
2.3 MBAdobe PDFView/Open
Show full item record Statistics


Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.

Sherman Centre for Digital Scholarship     McMaster University Libraries
©2022 McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L8 | 905-525-9140 | Contact Us | Terms of Use & Privacy Policy | Feedback

Report Accessibility Issue