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http://hdl.handle.net/11375/7080
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DC Field | Value | Language |
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dc.contributor.advisor | Andrews, David W. | en_US |
dc.contributor.author | Young, Jason C. | en_US |
dc.date.accessioned | 2014-06-18T16:38:00Z | - |
dc.date.available | 2014-06-18T16:38:00Z | - |
dc.date.created | 2010-06-29 | en_US |
dc.date.issued | 1995-12 | en_US |
dc.identifier.other | opendissertations/2376 | en_US |
dc.identifier.other | 3354 | en_US |
dc.identifier.other | 1375528 | en_US |
dc.identifier.uri | http://hdl.handle.net/11375/7080 | - |
dc.description.abstract | <p>The α subunit of the signal recognition particle receptor (SRα) is targeted to the endoplasmic reticulum membrane by a mechanism independent of the signal recognition particle-mediated translocation pathway. The membrane binding of SRα polypeptides was examined using a cell-free translation and targeting system. A membrane binding domain consisting of the amino-terminal 140 residues of SRα was identified. This domain forms a protease-resistant folded unit, and is the major site of tight binding to the β subunit of the receptor (SRβ). SRα was also shown to be a peripheral membrane protein, suggesting that it is bound to the membrane largely via interactions with the integral membrane SRβ. Co-translational targeting of SRα was correlated with a translation pause site on the SRα mRNA. An mRNA sequence at this pause site that resembles a class of retroviral frameshift sites was shown to be necessary and sufficient for a strong translation pause. SRα polypeptides synthesized from a mutant non-pausing mRNA were impaired in co-translational membrane binding, and translation-dependent localization of polysomes synthesizing SRα. These data resolve a discrepancy in earlier reports concerning the membrane attachment of SRα. Moreover, a novel pathway for polypeptide targeting and mRNA localization is proposed, and a previously undescribed signal for translation pausing is identified. Translation pausing may be general mechanism to coordinate co-translational folding, oligomeric assembly and targeting of other proteins.</p> | en_US |
dc.subject | Biochemistry | en_US |
dc.subject | Biochemistry | en_US |
dc.title | Membrane Assembly and Translation Pausing of the Signal Recognition Particle Receptor α Subunit | en_US |
dc.type | thesis | en_US |
dc.contributor.department | Biochemistry | en_US |
dc.description.degree | Doctor of Philosophy (PhD) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
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File | Size | Format | |
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fulltext.pdf | 2.93 MB | Adobe PDF | View/Open |
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