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Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/30672
Title: Commentary: Tracing the fate of metabolic substrates during changes in whole-body energy expenditure in mice
Authors: Lyons SA
McClelland GB
Department: Biology
Keywords: 30 Agricultural, Veterinary and Food Sciences;3101 Biochemistry and Cell Biology;31 Biological Sciences;3109 Zoology;3005 Fisheries Sciences;Nutrition;Prevention;Animals;Mice;Energy Metabolism;Fatty Acids;Physical Conditioning, Animal
Publication Date: Oct-2024
Publisher: Elsevier
Abstract: For small mammals, such as mice, cannulation procedures can be quite challenging, limiting research associated with tracing isotopically labelled substrates at the whole-animal level. When cannulation in mice is possible, assessment of substrate use is further limited to when mice are either under anesthesia or are at rest, as there are no studies directly quantifying substrate use during exercise in mice. The use of isotopic tracer techniques has greatly advanced our knowledge in understanding how metabolic substrates (carbohydrates, amino acids, and fatty acids) contribute to whole-body metabolism. However, research regarding tissue-specific fuel use contributions to whole-body energy expenditure in mice at varying metabolic intensities (i.e., exercise) is lacking, despite the popularity of using mice in a variety of metabolic models. In this commentary, we briefly discuss the methodologies, advantages, and disadvantages of using radiolabelled, positron emission, and stable isotopes with a specific focus on fatty acids. We highlight recent mouse studies that have used creative experimental designs employing the use of isotopic tracer techniques and we briefly discuss how these methodologies can be further pursued to deepen our understanding of substrate use during exercise. Lastly, we show findings of a recent study we performed using a radiolabelled fatty acid tracer (14C-bromopalmitic acid) to determine fatty acid uptake in 16 muscles, two brown and two white adipose tissue depots during submaximal exercise in deer mice.
URI: http://hdl.handle.net/11375/30672
metadata.dc.identifier.doi: https://doi.org/10.1016/j.cbpb.2024.111008
ISSN: 1096-4959
1879-1107
Appears in Collections:Biology Publications

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