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http://hdl.handle.net/11375/30112
Title: | REDUCE, REUSE, RECYCLE: EVALUATING THE CONTRIBUTION OF EUTREMA SALSUGINEUM BYPASSES FOR GLYCOLYTIC SUBSTRATE LEVEL PHOSPHORYLATION IN RESPONSE TO LOW PHOSPHATE CONDITIONS |
Authors: | MacLean, Madeline |
Department: | Biology |
Publication Date: | 24-Apr-2024 |
Publisher: | n/a |
Abstract: | Drought reduces the availability of dissolved nutrients for plant uptake. For the essential macronutrient phosphate (Pi), drought exacerbates its already low soil availability for plant uptake. Understanding the mechanisms allowing plants to thrive under semi-arid, low-Pi conditions enables us to develop climate change resilient crops less reliant on added Pi fertilizers. Eutrema salsugineum is an extremophile plant and the Yukon ecotype thrives on low-Pi soil. Yukon plants can bypass glycolytic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) using NADH and Pi to phosphorylate ADP by employing a non-phosphorylating (NP) GAPDH. By avoiding phosphorylation, NP-GAPDH can preserve Pi for alternative use in a deficient plant. The Shandong ecotype is less tolerant of low Pi, so their capacity to use the bypass was also evaluated. GAPDH and NP-GAPDH activity was measured in leaf and root cell-free extracts of plants grown with (+Pi) or without Pi (-Pi). For both ecotypes, up to 10-fold higher activity rates were found in leaf extracts relative to root extracts. In -Pi plants, leaf GAPDH activity was approximately three-fold and two-fold greater relative to +Pi plants for Yukon and Shandong respectively. Leaf NP-GAPDH activity was slightly greater in +Pi relative to -Pi in Yukon plants, while rates were comparable for -Pi and +Pi Shandong plants. Furthermore, NP-GAPDH activity in Yukon and Shandong plants were similar, suggesting that the bypass does not play an essential role for tolerance to low-Pi conditions and raises a question about the true metabolic role of this enzyme in plants. |
URI: | http://hdl.handle.net/11375/30112 |
Appears in Collections: | Student Publications (Not Graduate Theses) |
Files in This Item:
File | Description | Size | Format | |
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MacLean_Madeline_2024.pdf | 643.95 kB | Adobe PDF | View/Open |
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