Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/29732
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Rajabzadeh, Amin Reza | - |
dc.contributor.advisor | Srinivasan, Seshasai | - |
dc.contributor.author | Sherazee, Masoomeh | - |
dc.date.accessioned | 2024-05-04T01:05:39Z | - |
dc.date.available | 2024-05-04T01:05:39Z | - |
dc.date.issued | 2024 | - |
dc.identifier.uri | http://hdl.handle.net/11375/29732 | - |
dc.description.abstract | Over the past few decades, the use of colorimetric testing has been declining for reasons such as low sensitivity and slow incubation times. Scientists have attempted to remedy this by utilizing nanomaterials to provide enzymatic activity forcing the reaction to increase targeted binding and speed. The ability of a nanomaterial to imitate enzymatic activity is referred to as nanozymatic activity. In this thesis, firstly, a new, easy and quick synthesis method for Carbon Quantum Dot-Gold Nanoparticles (CQD-AuNPs) is conveyed. This nanohybrid is then used as a peroxidase-mimic in the oxidation reaction between 3,3′,5,5′-Tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) where it successfully catalyzed the reaction. This nanozyme was added to reaction mixtures for H2O2 and dopamine (DA) detection and produced incredibly low limit-of-detection (LOD) values alongside increased colorimetric intensity. Furthermore, the nanozyme’s behaviour under an externally applied electric potential was examined. Doing so severely decreased the incubation time and enhanced the detection of H2O2 and DA, thus procuring LOD values as low as 240 nM, and 0.75 mM, respectively. Secondly, new characterization tests were conducted on silicene nanosheets. Formerly, silicene was a material that was studied primarily via theoretical modelling. This thesis utilizes silicene’s exceptional semiconducting properties to promote colorimetric detection of H2O2 and DA. It was evaluated for its peroxidase-like activity in the oxidation reaction of TMB. Under an externally applied electric potential, the incubation time was reduced from 30 minutes to 1.5 minutes and presented lower LOD values - 3.11 M and 2.21M for H2O2 and DA, respectively. | en_US |
dc.language.iso | en | en_US |
dc.title | An Exploration of Enzyme-like Activity of Newly Synthesized Carbon Quantum Dot-Gold Nanoparticle Nanohybrids and Silicene Nanosheets for the Development of an Electrochemically Supported Colorimetric Biosensing Approach for the Detection of H2O2 and Dopamine. | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biomedical Engineering | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Applied Science (MASc) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sherazee_Masoomeh_2024April_MASc.pdf | 3.17 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.