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Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/29008
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dc.contributor.advisorWest-Mays, Judith-
dc.contributor.authorShirazee, Fatima-
dc.date.accessioned2023-10-06T18:03:55Z-
dc.date.available2023-10-06T18:03:55Z-
dc.date.issued2023-
dc.identifier.urihttp://hdl.handle.net/11375/29008-
dc.description.abstractThis project explores the use of a novel sustained release mucoadhesive micelle-based drug delivery system in combination with 0.005% latanoprost (LTP) on our partially open angle mouse model of glaucoma (AP-2β TMR-KO). We previously tested for LTP treatment in our model and found a reduction in intraocular pressure (IOP) 20 minutes following treatment. This information led us to investigate the long-term effect of LTP treatment and micelle loaded with LTP (MLTP) treatment in our model. We hypothesized that the MLTP treatment would be more effective in reducing IOP and preventing glaucomatous effects than LTP treatment alone in the AP-2β TMR-KO mice. The MLTP groups of animals (wildtype and mutant) were treated every 3 days, and this was compared with animals treated with LTP daily as well as animals treated every 3 days with LTP alone for comparison’s sake for 60 days. IOP measurements were taken every 3 days. Following long term LTP treatment alone, mutant mice showed a consistent decrease in their baseline IOPs with a significant reduction in baseline IOP at 35 days of treatment across all cohorts (P<0.0001). In comparison, mutants treated with MLTP exhibited an even greater reduction in baseline IOP following long term treatment. After the treatment period, mice were euthanized, and their eyes were enucleated, fixed, sectioned, and stained for retinal ganglion cells (RGCs) using Brn3a. Mutant mice exhibited a significant decrease in RGC cell number when compared to wildtype, and this loss was not rescued by treatment with LTP. However, mutants treated with MLTP demonstrated significant RGC cell protection compared to eyes of untreated mutants, as well as everyday LTP treated mutants.en_US
dc.language.isoenen_US
dc.subjectDrug Deliveryen_US
dc.subjectGlaucomaen_US
dc.subjectMicelleen_US
dc.subjectIntraocular Pressureen_US
dc.subjectLatanoprosten_US
dc.subjectRetinal Ganglion Cellsen_US
dc.titleInvestigating the Effect of a Micelle-Based Drug Delivery System in Reducing IOP and Glaucomatous Effects in a Partially Open Angle Mouse Model of Glaucomaen_US
dc.typeThesisen_US
dc.contributor.departmentMedical Sciencesen_US
dc.description.degreetypeThesisen_US
dc.description.degreeMaster of Science (MSc)en_US
dc.description.layabstractAn effective treatment strategy is required to prevent irreversible blindness caused from glaucoma. Unfortunately, compliance with current medications is extremely poor, as they require frequent administration due to their low ocular bioavailability and short-term effect. As such, this thesis aims to explore an alternative drug delivery approach in a partially open angle mouse model of glaucoma to prevent the worsening of glaucoma and ultimately improve patient compliance.en_US
Appears in Collections:Open Access Dissertations and Theses

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