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Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/28451
Title: The Effect of Cannabinoids and Cannabis on Mammary Epithelial Cell Function and Breast Milk Composition
Other Titles: EFFECT OF CANNABIS ON MAMMARY EPITHELIAL CELLS AND BREAST MILK
Authors: Josan, Chitmandeep
Advisor: Raha, Sandeep
Department: Medical Sciences
Keywords: Mammary Gland;ER Stress;Cannabis;Breast milk;Lactation;Mammary epithelial cells;THC;CBD;milk protein;lipid
Publication Date: 2023
Abstract: Mammary gland (MG) is a dynamic organ that is essential for the production and secretion of breast milk. During pregnancy the MG undergoes a critical phase of remodeling, which is accompanied by the differentiation of mammary epithelial cells (MECs). During lactation, the MG requires high level of energy for proper folding of proteins in the secretory pathway, which takes place in the endoplasmic reticulum (ER). Limited evidence has been reported on the impact of cannabis or its components, delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD), on the differentiation of MECs. However, both THC and CBD have been reported to induce ER stress in various cell types, resulting in impacting cellular function. Furthermore, consequences of cannabis use in the perinatal period on breast milk composition have not been reported. Using the HC11 cell line, we investigated whether THC and CBD evoke ER stress in MECs resulting in the impaired cellular function. We also reported on the effects of cannabis use during pregnancy and lactation on the levels of macronutrients and bioactive factors in the breast milk of cannabis users. Relative to control, 10μM THC and 10μM CBD reduced mRNA levels of milk proteins (CSN2 and WAP) and lipid synthesizing enzymes (FASN, FABP4, PLIN2 and LPL), as well as whey acidic protein and lipid levels. In addition, 10μM THC, 10μM CBD, and the combination of 10μM THC + 10μM CBD significantly induced the expression of ER stress genes in HC11 cells. Furthermore, in the milk of women who used cannabis during pregnancy and lactation, the levels of secretory immunoglobulin A (SIgA) were decreased, relative to non-users. Altogether, our findings indicate that cannabis use in the perinatal period may have implications on breast milk composition and infant health.
URI: http://hdl.handle.net/11375/28451
Appears in Collections:Open Access Dissertations and Theses

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