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http://hdl.handle.net/11375/28290
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DC Field | Value | Language |
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dc.contributor.advisor | Surette, Michael G. | - |
dc.contributor.author | Ixtepan Tejero, Claudia | - |
dc.date.accessioned | 2023-01-30T20:10:45Z | - |
dc.date.available | 2023-01-30T20:10:45Z | - |
dc.date.issued | 2023 | - |
dc.identifier.uri | http://hdl.handle.net/11375/28290 | - |
dc.description.abstract | Glycans, carbohydrate-based macromolecules, are present on the surfaces of all prokaryotes and eukaryotes cells. They play critical roles in cell-cell interactions, and host glycans also provide both binding sites and nutrition for commensal microbes. Many bacteria use glycoside hydrolases (GHs) on host-generated glycans and dietary glycans for nutrition and to modulate attachment sites and host protein function. The microbiota found in a single human gut sample has greater than 10,000 GH genes, and many of these gene products have specificity for host glycans. In eukaryotes, glycosylation of cell surface receptors is critical for proper localization and function, including receptors for innate immune signalling. The hypothesis of this project is that human-associated microbiota can express and secrete GHs that can alter the functioning of host immune signalling pathways. In this work, I measure GHs enzyme activity, use bacterial cell-free supernatant and purified enzymes to determine if they can modulate host cell signalling pathways, and examine glycans cell surface modifications by fluorescence microscopy using fluorescent-labelled lectins. | en_US |
dc.language.iso | en | en_US |
dc.subject | GHs, Microbiome, Glycans, TLRs, | en_US |
dc.title | Microbial Glycoside Hydrolases and Host Cell Signaling | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biochemistry and Biomedical Sciences | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MSc) | en_US |
dc.description.layabstract | Glycans are sugars that cover cells of animals, plants, and microbes. These sugars on the external surface of the cell help them interact with and protect them from the environment. For our microbiomes – the microbes that live on or in us- glycans on both microbe and our cells are important for interactions between them and us. Many bacteria produce enzymes (such as glycoside hydrolases) that change or remove sugars from the cell surface to obtain nutrients and manipulate host proteins. These enzymes can be attached to the bacterial cell surface or released into the environment, where they can interact with glycans attached to mucosal surface and immune cells. The modification of cell glycans from the immune system can modulate inflammatory responses that could change the way gut cells react to certain kinds of food, such as legumes and wheat. My work focuses on understanding how human gut microbiome can use these tools to reshape glycans and their effects on our health. | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Ixtepan Tejero_Claudia_202301_MSc.pdf | 2.24 MB | Adobe PDF | View/Open |
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