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Title: | DEVELOPMENT OF MEMBRANE CHROMATOGRAPHIC SEPARATION AND THEIR APPLICATION FOR BIOPHARMACEUTICAL (THERAPEUTIC MONOCLONAL ANTIBODIES AND T7 BACTERIOPHAGE) PURIFICATION |
Authors: | Roxana Roshankhah |
Advisor: | Dr. Raja Ghosh, Dr. Robert Pelton |
Department: | Chemical Engineering |
Publication Date: | 2022 |
Abstract: | Biopharmaceuticals, produced and extracted from living cells and organisms, are targeted treatments for a variety of medical conditions and diseases, some of which have no other available treatments. The manufacturing of biopharmaceuticals consists of upstream and downstream processes. The downstream operation includes several steps, such as purification and quality controls, among others. The purification process is crucial in the production of biopharmaceuticals, as a high impurity rate renders the treatment unusable. The production of therapeutic biopharmaceuticals is bounded by the efficiency of the purification process. Due to its close connection to the purification process, industrial solutions such as Chromatography purification became significantly important, with several qualitative and quantitative techniques for mixture components identification, separation, and purification, was introduced and adopted within this field. In this thesis, the Trastuzumab purification process is investigated using cation-exchange z2 Laterally-Fed Membrane Chromatography (z2LFMC) purification technique. z2LFMC in a purification device that leverages z2 flow distribution characteristics to enhance the separation efficiency of a laterally-fed membrane chromatography. Exploring the performance discrepancy between z2LFMC and affinity chromatography (Considers as one of the most diverse and powerful chromatographic methods for purification) in the process of Trastuzumab purification. The experimental evaluation highlights that both chromatography methods (z2LFMC and protein A) achieve more than 95% purity efficiency. However, the recovery rate of z2LFMC was noticeably higher (90.4% in comparison with 81.7%). Bacteriophages were widely used to treat various bacterial diseases in people and animals. Further, the purification of T7 bacteriophage membrane chromatography by anion exchange z2LFMC shows a promising research direction. The results show that following anion exchange z2LFMC enables a recovery rate of up to 89.0%, and a 71.2% endotoxin removal rate. This thesis further explores the potential enhancements in the separation process. In particular, Computational Fluid Dynamic (CFD) modeling is utilized toward developing several design variations of the z2LFMC. This thesis highlights that the biopharmaceuticals purification process is nowhere optimized, and several state-of-the-art approaches, including z2LFMC, can be used to significantly increase the efficiency of the purification process. |
URI: | http://hdl.handle.net/11375/27674 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Roshankhah_Roxana_finalsubmission2022JUNE_MASc.pdf | 2.03 MB | Adobe PDF | View/Open |
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