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http://hdl.handle.net/11375/26506
Title: | Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 |
Authors: | Banerjee A Nasir JA Budylowski P Yip L Aftanas P Christie N Ghalami A Baid K Raphenya AR Hirota JA Miller MS McGeer AJ Ostrowski M Kozak RA McArthur AG Mossman K Mubareka S |
Keywords: | 2019 novel coronavirus disease;COVID-19;SARS-CoV-2;coronavirus disease;immune cells;isolation;phylogenetics;replication;respiratory infections;severe acute respiratory syndrome coronavirus 2;viruses;zoonoses;Betacoronavirus;COVID-19;Coronavirus Infections;DNA, Viral;Genotype;Humans;Kinetics;Leukocytes, Mononuclear;Pandemics;Pneumonia, Viral;Polymorphism, Single Nucleotide;SARS-CoV-2;Virus Replication;Whole Genome Sequencing |
Publication Date: | Sep-2020 |
Publisher: | Centers for Disease Control and Prevention (CDC) |
Abstract: | Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models. |
metadata.dc.rights.license: | Attribution - CC BY |
Rights: | Attribution - CC BY This Creative Commons license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. Recommended for maximum dissemination and use of licensed materials. |
URI: | http://hdl.handle.net/11375/26506 |
metadata.dc.identifier.doi: | https://doi.org/10.3201/eid2609.201495 |
ISSN: | 1080-6040 1080-6059 |
Appears in Collections: | Faculty Publications (via McMaster Experts) |
Files in This Item:
File | Description | Size | Format | |
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Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2.pdf | Published version | 3.72 MB | Adobe PDF | View/Open |
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