Investigating the Role of LAMP3 in HIV-1 Uptake and Transcytosis Across Vaginal Epithelial Cells

Abstract

It is estimated that 38 million people currently live with HIV-1. Of the 38 million currently infected, 19.2 million women are infected compared to 17 million men, indicating a clear disproportion. As such, understanding mechanisms that result in increased susceptibly in women is critical to develop more efficacious prevention strategies. The epithelial cells that line the lower female reproductive tract are the first line of defense against invading pathogens. The current consensus in the field is that HIV-1 can cross the mucosal epithelium in two ways: paracellular passage and transcytosis. There are several endocytic pathway associated proteins within vaginal epithelial cells that may have a role in viral transcytosis, however, little is known about their role. One of these is Lysosomal Associated Membrane Protein 3 (LAMP3), which has been shown to be upregulated following viral exposure and involved in viral trafficking. The mechanisms regarding the early events of transmission of HIV-1 across vaginal epithelial cells remains unclear and warrant further investigation. This study was designed to examine the mechanism of how HIV1 crosses vaginal epithelial cells and potential interactions of LAMP3 and HIV-1.