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http://hdl.handle.net/11375/25190
Title: | Identifying Novel Regulatory Inputs Governing Salmonella Enterica Niche-Specific Gene Expression |
Other Titles: | Niche Specific Gene Regulation in Salmonella Enterica |
Authors: | Ilyas, Bushra |
Advisor: | Coombes, Brian |
Department: | Biochemistry and Biomedical Sciences |
Keywords: | Salmonella;Regulatory Evolution;Bacterial pathogenesis;SsrA-SsrB |
Publication Date: | 2019 |
Abstract: | Salmonella enterica is an enteric pathogen with a broad host tropism that can cause disease ranging from self-limited gastroenteritis to enteric fever. The evolution of S. enterica as a pathogen is driven by the horizontal acquisition of genes that promote virulence and survival within host immune cells, as well as the coordinated regulation of these and ancestral genes by two-component systems (TCS). TCS integrate environmental cues with the transcriptional reprogramming of bacteria, and in the case of Salmonella, result in niche-specific gene expression in response to anti-bacterial cues produced by the host. The TCS SsrA-SsrB in S. enterica is considered the master regulator for intracellular virulence, where SsrA is a sensor kinase that triggers the activation of the DNA binding protein SsrB. The full suite of genes regulated by SsrB in S. enterica, as well as the cues that activate this TCS, have not been fully characterized. Here, we demonstrated that horizontally acquired and ancestral genes in the S. enterica genome have evolved to be regulated by SsrB, and the repression of a set of ancestral genes involved in flagellar motility promotes evasion of the host immune system. Additionally, we identified the production of reactive oxygen species (ROS) by host immune cells as a signal that can activate a cluster of genes regulated by the SsrA-SsrB TCS, likely mediated by SsrA sensing of these ROS. Together, these results expand our understanding of the complex interplay between the pathogen S. enterica and the host that results in bacterial infections. |
URI: | http://hdl.handle.net/11375/25190 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Ilyas_Bushra_2019April_PhD.pdf | 7.76 MB | Adobe PDF | View/Open |
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