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|Title:||Effects of low-dose ionizing radiation in utero on postnatal growth and cardiovascular physiology in BALB/cJ mice|
|Keywords:||Ionizing radiation;Fetal Programming;Blood Pressure;Cardiovascular|
|Abstract:||Diagnostic radiation is typically avoided during pregnancy, as the effect of low-dose radiation exposure on the fetus is uncertain. The objective of this study was to determine if ionizing radiation exposure during late fetal development would cause an adverse intrauterine environment, and lead to growth restriction of offspring and a hypertensive phenotype later in life. To study this, pregnant BALB/cJ mice were exposed to ionizing radiation at 5, 10, 50, 100, 300 or 1000 mGy on gestational day 15. Offspring were weighed weekly from the age of weaning until a mature age of 16 weeks. Cardiovascular effects were assessed every other week via heart rate and blood pressure measurements using tail plethysmography. The expression of genetic markers for endothelial dysfunction, inflammation, mitochondrial capacity, and regulation of the oxidative stress response in the aorta and heart for the 1000 mGy was assessed from tissue collected at 17 weeks of age. We observed no effects of low to mid dose (5-300 mGy) radiation on offspring growth and blood pressure. Growth restriction was observed in male and female offspring exposed to high-dose radiation (1000 mGy). In the heart, there was no observed effect on mitochondrial capacity and oxidative stress response genes. In the aorta, we observed decreased TNF-α expression in male offspring, which may be linked to the growth restriction but was not considered a sign of cardiovascular dysfunction. There were no observed effects of exposure to 1000 mGy on cardiovascular function. This study provides knowledge on the possible effects of radiation on in utero development, which broadens the knowledge on the range of stressors capable of affecting offspring growth and development.|
|Appears in Collections:||Open Access Dissertations and Theses|
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|Preston_Jessica_M_2019-09_MSc.pdf||1.11 MB||Adobe PDF||View/Open|
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