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http://hdl.handle.net/11375/24938
Title: | The role of resting mast cells in the survival of myenteric neurons |
Other Titles: | The role of resting mast cells in the survival of myenteric neurons in a primary longitudinal muscle-myenteric plexus & bone marrow-derived mast cell co-culture system |
Authors: | Knoch, Jaime |
Advisor: | Forsythe, Paul |
Department: | Medical Sciences |
Keywords: | Enteric nervous system;Mast cells;Myenteric plexus;BMMC;Excitotoxicity;Kynurenine Pathway |
Publication Date: | 2019 |
Abstract: | The enteric nervous system (ENS) is an incredibly complex neural network that is extensively integrated within the neuroimmunoendocrine system through countless signalling pathways that have yet to be fully characterized. In the last decade we have discovered that many more neurotransmitters are at work in the ENS than was originally thought. This opens up new avenues of research into physiological phenomena traditionally thought to be associated only with the central nervous system, such as NMDA receptor-induced excitotoxicity, and how these may influence immune interactions. In particular, the kynurenine pathway of the tryptophan catabolism produces many neuro-active and immuno-active constituents whose effects are unknown in the ENS but are of great consequence in many neurodegenerative disorders of the CNS. Our study hypothesized that co-culture of the enteric neurons with mast cells would increase neuronal survival through kynurenic acid production in quinolinic acid (QUIN)-induced excitotoxic conditions. This study developed a novel in vitro co-culture system of enteric neurons and glia grown from murine longitudinal muscle-myenteric plexus tissue and bone marrow-derived mast cells. In addition, a pipeline in image analysis software CellProfiler was designed and optimized in order to reduce human bias and error in subsequent immunocytochemical image analysis. Furthermore, we identified the genetic expression of subunits of the NMDA glutamate receptor in cultured enteric neurons via PCR, which suggests that these cultured neurons may be susceptible to excitotoxicity. PCR analysis of cultured mast cells seemed to indicate that our cultured mast cells do not express KAT-III, the enzyme needed to produce the neuroprotective KYNA. Overall, co-culture with mast cells seemed to decrease neuronal survival. This project developed a novel methodology for the in vivo study of mast cell-nerve interactions, and lays the groundwork for future studies in excitotoxicity in the ENS. |
URI: | http://hdl.handle.net/11375/24938 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Knoch_Jaime_K_2019Oct_MSc.pdf | 6.94 MB | Adobe PDF | View/Open |
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