Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/23673
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Li, Yingfu | - |
dc.contributor.author | Cruz, Rani Priya Gomez | - |
dc.date.accessioned | 2018-12-19T02:27:08Z | - |
dc.date.available | 2018-12-19T02:27:08Z | - |
dc.date.issued | 2003-12 | - |
dc.identifier.uri | http://hdl.handle.net/11375/23673 | - |
dc.description.abstract | We conducted 16 parallel in vitro selection experiments to isolate catalytic DNAs from a common DNA library for the cleavage of all 16 possible dinucleotide junctions of RNA incorporated into a common DNA/RNA chimeric substrate sequence. We discovered hundreds of sequence variations of the 8-17 deoxyribozyme - an RNA-cleaving catalytic DNA motif previously reported - from nearly all 16 final pools. Sequence analyses identified four absolutely conserved nucleotides in 8-17. Five representative 8-17 variants were tested for substrate cleavage in trans and together they were able to cleave 14 dinucleotide junctions. New 8-17 variants required Mn2+ to support their broad dinucleotide cleavage capabilities. We hypothesize that 8-17 has a tertiary structure composed of an enzymatic core executing catalysis and a structural facilitator providing structural fine-tuning when different dinucleotide junctions are given as cleavage sites. | en_US |
dc.language.iso | en | en_US |
dc.subject | 8-17 deoxyribozyme | en_US |
dc.subject | dinucleotide junction | en_US |
dc.title | Dinucleotide Junction Cleavage Versatility of 8-17 Deoxyribozyme | en_US |
dc.title.alternative | Cleavage Versatility of 8-17 Deoxyribozyme | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biochemistry | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MSc) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cruz_Rani_Priya_Gomez_2003Dec_Masters.pdf | 21.96 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.