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http://hdl.handle.net/11375/23673
Title: | Dinucleotide Junction Cleavage Versatility of 8-17 Deoxyribozyme |
Other Titles: | Cleavage Versatility of 8-17 Deoxyribozyme |
Authors: | Cruz, Rani Priya Gomez |
Advisor: | Li, Yingfu |
Department: | Biochemistry |
Keywords: | 8-17 deoxyribozyme;dinucleotide junction |
Publication Date: | Dec-2003 |
Abstract: | We conducted 16 parallel in vitro selection experiments to isolate catalytic DNAs from a common DNA library for the cleavage of all 16 possible dinucleotide junctions of RNA incorporated into a common DNA/RNA chimeric substrate sequence. We discovered hundreds of sequence variations of the 8-17 deoxyribozyme - an RNA-cleaving catalytic DNA motif previously reported - from nearly all 16 final pools. Sequence analyses identified four absolutely conserved nucleotides in 8-17. Five representative 8-17 variants were tested for substrate cleavage in trans and together they were able to cleave 14 dinucleotide junctions. New 8-17 variants required Mn2+ to support their broad dinucleotide cleavage capabilities. We hypothesize that 8-17 has a tertiary structure composed of an enzymatic core executing catalysis and a structural facilitator providing structural fine-tuning when different dinucleotide junctions are given as cleavage sites. |
URI: | http://hdl.handle.net/11375/23673 |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Cruz_Rani_Priya_Gomez_2003Dec_Masters.pdf | 21.96 MB | Adobe PDF | View/Open |
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