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http://hdl.handle.net/11375/23300
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DC Field | Value | Language |
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dc.contributor.advisor | Smiley, J. R. | - |
dc.contributor.author | Davies, Sherry | - |
dc.date.accessioned | 2018-08-22T14:16:55Z | - |
dc.date.available | 2018-08-22T14:16:55Z | - |
dc.date.issued | 1986 | - |
dc.identifier.uri | http://hdl.handle.net/11375/23300 | - |
dc.description.abstract | Herpes simplex virus (HSV) genes are expressed as at least three coordinately regulated gene classes during lytic infection. The delayed-early (DE) and late (L) genes require previous expression of one or more immediate-early (IE) genes for their own expression. The DE genes achieve maximal expression prior to viral DNA synthesis, while the L genes are maximally expressed after DNA replication (Honess and Roizman, 1974). A recombinant strain of HSV-1, X1N17, was used in this study to examine the effect of the gene promoter on the temporal expression of HSV genes. This virus carries a late viral promoter upstream from the coding sequences of a DE gene (thymidine kinase; TK). S1-mapping studies showed that X1N17-TK transcripts initiated under the control of the late promoter and accumulated with L class kinetics. However, the TK activity levels in X1N17-infected cells were not consistent with HSV late gene expression. Western blot analysis of infected cell proteins revealed that despite the high levels of X1N17-TK mRNA present in the cytoplasm late after infection, little TK polypeptide was being synthesized. This suggested that HSV genes are subject to post-transcriptional control mechanisms that modulate the efficiency of translation of viral transcripts. More specifically, it appears as though HSV-TK transcripts are not efficiently translated at late times in infected cells. | en_US |
dc.language.iso | en | en_US |
dc.subject | herpes simplex virus | en_US |
dc.subject | herpes | en_US |
dc.subject | thymidine | en_US |
dc.subject | gene | en_US |
dc.subject | gene expression | en_US |
dc.subject | viral promoter | en_US |
dc.subject | control | en_US |
dc.title | Herpes Simplex Virus Thymidine Kinase Gene Expression Under Control of a Late Viral Promoter | en_US |
dc.title.alternative | Post-Transcriptional Regulation of HSV Thymidine Kinase Expression | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biology | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MS) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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davies_sherry_1986_masters.pdf.pdf | 13.32 MB | Adobe PDF | View/Open |
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