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http://hdl.handle.net/11375/23161
Title: | Signalling Pathways Regulating BC₃H1 Cell Myogenesis |
Authors: | Oakes, Janice |
Advisor: | Lee, R. W. H. |
Department: | Biology |
Keywords: | signal;pathway;BC₃H1;cell;myogenesis |
Publication Date: | 1991 |
Abstract: | The myogenic cell line, BC₃H1, upon cell-to-cell contact or serum starvation differentiates as monitored by the appearance of muscle-specific markers, actin, myosin light chain 1 (MLC 1) and tropomyosin (Tm) and morphological changes. The detection of MLC 1 and five Tm isoforms in this cell line is novel. To assess the role of protein kinase C (pk C)-and protein kinase A (pk A) signal transduction pathways in controlling BC₃H1 cell differentiation, activators of pk C (TPA) and pk A (cAMP analogues, dibutyryl-cAMP and 8-Br-cAMP) were used. TPA (500nM) addition caused no deviation from the normal expression patterns of actin, Tm and MLC 1. Addition of cAMP analogues (500μM) delayed the appearance of MLC 1 and muscle-specific isoforms of Tm, as well as α-actin while β- and γ-actin levels remained unchanged. However, α-actin mRNA levels were not affected by cAMP analogues yet the typical β- and γ-actin mRNA downregulation was blocked. cAMP appears to be operating at multiple levels to regulate BC₃H1 cell myogenesis such as post-transcriptional and translational. In addition, given the similarity in mechanisms through which cAMP and adenovirus early region 1A (AdE1A) mediate gene activation, the effect of AdE1A on BC₃H1 cell differentiation was investigated. A stable transfected AdE1A clonal cell line, BC₃E7, was characterized. Together with altered morphology, BC₃E7 cells failed to show the characteristic expression of muscle-specific markers actin, Tm and MLC 1. AdE1A transfection disrupted the synchronous expression of muscle-specific proteins during BC₃H1 cell differentiation. |
URI: | http://hdl.handle.net/11375/23161 |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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oakes_janice_d_1991_masters.pdf | 9.69 MB | Adobe PDF | View/Open |
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