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|Title:||The Ability of Human Adenovirus Type 5 to Replicate in MDBK, ADCK, HELA & L Cells|
|Other Titles:||The Replication of hAd5 in MDBK, MDCK, HELA & L Cells|
|Abstract:||Human adenovirus type 5 (hAd5) may be used as an effective mammalian expression vector for foreign genes. In the course of determining the usefulness of hAd5 as a vector in different species, it was observed that hAd5 does not undergo a productive infection in canine kidney cells 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 (unpublished observations), though it produces an immune response 𝘪𝘯 𝘷𝘪𝘷𝘰 (Prevec et al., 1990). Murine fibroblasts (L cells) are semi-permissive for hAd5 replication, with DNA being synthesized at low levels, and the E3 region displaying delayed kinetics for expression when compared to permissive infections of HeLa cells. Bovine kidney cells (MDBK) display a similar rate of hAd5 replication as in HeLa cells, although infectious virus yield is somewhat lower in MDBK cells than in HeLa cells; this may be in part due to a decreased level in late protein synthesis. Canine kindey cells (MDCK) appear non-permissive for hAd5, with early proteins being synthesized, DNA replication occurring at lower levels, and no late proteins being produced. Infection of MDCK cells with Ad5lacZ resulted in similar rates of β-galactosidase expression as in permissive HeLa cells, suggesting that high levels of early protein expression can be achieved without virus replication. Although no late proteins are made, at least one late transcript (penton base) was detected in the cytoplasm of infected MDCK cells, which may suggest a block in translation initiation and/or elongation of late transcripts.|
|Appears in Collections:||Digitized Open Access Dissertations and Theses|
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