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http://hdl.handle.net/11375/22979
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DC Field | Value | Language |
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dc.contributor.advisor | Smiley, James | - |
dc.contributor.author | Koop, Karen | - |
dc.date.accessioned | 2018-05-29T13:16:26Z | - |
dc.date.available | 2018-05-29T13:16:26Z | - |
dc.date.issued | 1994-04 | - |
dc.identifier.uri | http://hdl.handle.net/11375/22979 | - |
dc.description.abstract | Herpes simplex virus is an important model in the study of temporally regulated gene expression in eukaryotic cells. Three classes of genes - immediate early, early, and late - are sequentially expressed during the course of lytic infection. One immediate early gene product, ICP4, is required for transactivation of most early and late genes; it is also implicated in repression of immediate early gene expression. ICP4's mechanism(s) of action is/are not yet understood; although ICP4 binds to specific sequences of DNA, whether this is necessary for transregulation by ICP4 is not clear. To gain a better understanding of how the ability of ICP4 to bind DNA relates to its transregulatory activities, I introduced ICP4 binding sites into a simple model promoter within the viral genome. Two sets of construct were made in which an ICP4 binding site (or mutant site) was placed either downstream or upstream of a TATA box, reproducing the spacing found in (i) the native 𝘐𝘊𝘗4 promoter and (ii) the native 𝘐𝘊𝘗0 promoter (respectively). The promoter of HSV-1 𝘜𝘓24𝘣 (a nonessential gene in tissue culture) was replaced with these model promoters and levels of transcripts accumulating from these constructs during lytic infection assayed by primer extension. I found that an ICP4 site placed either upstream or downstream of a TATA box shifted kinetics of expression from E/leaky L to true L. Neither the strength of the TATA box nor the helical orientation of the ICP4 binding site with respect to the TATA box affected this result. | en_US |
dc.language.iso | en | en_US |
dc.subject | DNA | en_US |
dc.subject | HSV-1 | en_US |
dc.subject | gene | en_US |
dc.subject | expression | en_US |
dc.title | The Function of DNA Binding in the Regulation of HSV-1 Gene Expression by ICP4 | en_US |
dc.title.alternative | Regulation of HSV-1 Gene Expression by ICP4 | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biology | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MS) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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koop_karen_1994Apr_masters.pdf | 14.84 MB | Adobe PDF | View/Open |
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