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http://hdl.handle.net/11375/22949
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DC Field | Value | Language |
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dc.contributor.advisor | McCalla, D. R. | - |
dc.contributor.author | Orr, Joan | - |
dc.date.accessioned | 2018-05-17T18:06:29Z | - |
dc.date.available | 2018-05-17T18:06:29Z | - |
dc.date.issued | 1985-09 | - |
dc.identifier.uri | http://hdl.handle.net/11375/22949 | - |
dc.description.abstract | S. typhimurium strain TA98/1,8-DNP₆ is resistant to mutagenesis by 1,8-dinitropyrene and is deficient in an acetyl-CoA dependent acetyltransferase activity. Strains TA98 and TA98NR which are acetyltransferase competent are sensitive to 1,8-dinitropyrene mutagenesis. The coincidence of acetyltransferase deficiency and 1,8-dinitropyrene resistance in strain TA98/1,8-DNP₆ implicates acetylation as an important process in the metabolic activation of 1,8-dinitropyrene to a mutagenic intermediate. The acetyltransferase activity can be assayed by observing the formation of 1-N-acetylamino-8-aminopyrene and 1,8-N,N'-diacetyldiaminopyrene from 1,8-diaminopyrene. Reduction of the nitro-function is also an important enzymatic step involved in the activation of 1,8-dinitropyrene. Evidence is presented which suggests that a nitroreductase-acetyltransferase enzyme complex may exist. Further investigations of nitroreductase activity have yielded results which indicate that three distinct nitroreductases exist, one specific for 1,8-dinitropyrene, one specific for 1-nitropyrene and one specific for nitrofurazone. The implications of these findings are discussed and an explanatory model is proposed. | en_US |
dc.language.iso | en | en_US |
dc.subject | salmonella | en_US |
dc.subject | metabolism | en_US |
dc.title | The Metabolism of 1,8-Dinitropyrene by Salmonella typhimurium | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biochemistry | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MS) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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orr_joan_1985Sept_masters.pdf | 2.45 MB | Adobe PDF | View/Open |
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