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http://hdl.handle.net/11375/22936
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DC Field | Value | Language |
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dc.contributor.advisor | Finan, T. M. | - |
dc.contributor.author | Yarosh, Oksana | - |
dc.date.accessioned | 2018-05-15T14:26:57Z | - |
dc.date.available | 2018-05-15T14:26:57Z | - |
dc.date.issued | 1989-01 | - |
dc.identifier.uri | http://hdl.handle.net/11375/22936 | - |
dc.description.abstract | Rhizobium meliloti mutants defective in C₄-dicarboxylic acid transport (Dct⁻) were previously isolated by Tn5 mutagenesis, and divided into two groups based on complementation of Dct- with cosmid clones. In this work further characterization was carried out on the two loci. Group I mutants were found to be defective in dicarboxylate transport (Dct⁻), nitrate utilization, and symbiotic nitrogen fixation. Subcloning and complementation work confined the Group I mutations to a 3.5 kbp BamHI-EcoRI fragment containing the ntrA gene. Group V mutants were defective in dicarboxylate transport and demonstrated varying levels of nitrogen fixation. Complementation and site-directed Tn5 mutagenesis revealed three transcriptional units, corresponding to dctA, dctB, and dctD, localized within a 6 kbp HindIII fragment. The use of dctA::TnphoA fusions determined the expression of dctA to be ntrA, dctB, and dctD dependent. Dct+ revertants of dctB and dctD mutants were selected which carried second-site mutations responsible for restoring the Dct⁺ phenotype. | en_US |
dc.language.iso | en | en_US |
dc.subject | c4 | en_US |
dc.subject | dicarboxylic acid | en_US |
dc.subject | transport gene | en_US |
dc.subject | gene | en_US |
dc.subject | rhizobium meliloti | en_US |
dc.title | Analysis of C4-Dicarboxylic Acid Transport Genes in Rhizobium Meliloti | en_US |
dc.title.alternative | C4-Dicarboxylic Acid Transport Genes in R. Meliloti | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biology | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MS) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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yarosh_oksana_k_1989Jan_masters.pdf | 4.65 MB | Adobe PDF | View/Open |
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