Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/22906
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Berghuis, Albert | - |
dc.contributor.author | Schwartzenhauer, Jeff | - |
dc.date.accessioned | 2018-05-08T15:26:38Z | - |
dc.date.available | 2018-05-08T15:26:38Z | - |
dc.date.issued | 1999-11 | - |
dc.identifier.uri | http://hdl.handle.net/11375/22906 | - |
dc.description.abstract | Bacterial resistance to the aminoglycoside antibiotics is a major health concern because of the elimination of a therapeutic option for the treatment of nosocomial infections. Clinical resistance is commonly caused by the acquisition of genes that encode an aminoglycoside modifying enzyme. These enzymes offer a potential therapeutic target in the fight against aminoglycoside resistance. By gaining a structural understanding of these enzymes the potential is created for rational drug design. The research presented here deals with structural studies on two aminoglycoside resistance enzymes. First the initial stages of structural determination for the bifunctional Aminoglycoside 6'-N-Acetyl transferase Aminoglycoside 2''-O-Phosphotransferase (AAC(6')-APH(2")) including the optimization of the purification procedure for this enzyme. The second enzyme is the Aminoglycoside 3'-O-Phosphotransferase (APH(3')IIIa). Computational studies on this enzyme have been carried out in order to determine models for aminoglycoside binding and also to search for potential enzyme inhibitors. The molecular docking studies for both the aminoglycoside binding and inhibitor search involved the development of a number of novel methods to improve the chance of obtaining a correct model, and to aid in the analysis of the data from the docking studies. These methods have the potential to be applied in future structure based drug design | en_US |
dc.language.iso | en | en_US |
dc.subject | aminoglycoside | en_US |
dc.subject | enzyme | en_US |
dc.subject | drug | en_US |
dc.subject | rational | en_US |
dc.title | Structural Analysis of Aminoglycoside Modifying Enzymes: Towards Rational Drug Design | en_US |
dc.title.alternative | Structural Analysis of Aminoglycoside Modifying Enzymes | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biochemistry | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Master of Science (MS) | en_US |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
schwartzenhauer_jeff_1999Nov_masters.pdf | 10.15 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.