Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/22890
Title: | Electrochemical protein detection by target-responsive programmable dynamic DNA assembly |
Authors: | Hasan, Md Roqibul |
Advisor: | Soleymani, Dr. Leyla |
Department: | Biomedical Engineering |
Keywords: | Protein responsive strand displacement assay. |
Publication Date: | 2018 |
Abstract: | Nucleic acid amplification is responsible for pushing the limit-of-detection of molecular diagnostic assays to unprecedented levels. We developed an assay based on protein-responsive programmable dynamic DNA assembly (PRPDA) to detect proteins via an intermediate process involving nucleic acids for taking advantage of nucleic acid amplification strategies. PRPDA has previously been designed for sensitive protein analysis in fluorescent assay formats. To further push the detection limit and to achieve assay miniaturization and multiplexing, we sought to combine PRPDA with electrochemical readout. We were able to achieve LOD of 1 pM by employing wrinkled gold electrode for the PRDA protein detection scheme. Which is 2800 times improvement compare to the 2.8 nM demonstrated by fluorescent transduction. |
URI: | http://hdl.handle.net/11375/22890 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Md Roqibul Hasan_Dec_2017_M.A.Sc..pdf | 6.96 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.