Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/22027
Title: | NON-CODING RNAS AND MRNA SECONDARY STRUCTURE IN STREPTOMYCES |
Authors: | Moody, Matthew John |
Advisor: | Elliot, Marie A |
Department: | Biology |
Keywords: | bacteria;gene regulation;non-coding rna;streptomyces;quorum sensing;RNA structure |
Publication Date: | 2017 |
Abstract: | Work over the past two decades has revealed that non-coding RNAs (ncRNAs) are prevalent in all kingdoms of life. Using RNA-seq we discovered hundreds of ncRNAs in the antibiotic-producing genus of bacteria, Streptomyces. These included trans-encoded small RNAS (sRNAs), cis-antisense RNAs, and a new type of antisense RNA we termed cutoRNAs (convergent untranslated overlapping RNAs) that arise when transcription termination does not occur in the intergenic region between two convergently arranged genes. Many of these ncRNAs feature prominently in the specialized metabolite biosynthetic clusters (e.g. antibiotics, anticancer agents, immunosuppressants). Hence, it is likely that understanding the functions of these RNAs will be important for new molecule discovery. We found that one highly expressed antisense RNA (ScbN) was expressed opposite the -butyrolactone synthase scbA in the model streptomycete Streptomyces coelicolor. However, ScbN had no detectible impact on the expression of scbA. Instead, the transcription terminator of scbN, which also forms a hairpin within the coding sequence of scbA, was found to reduce expression of scbA more than 10-fold. This led us to bioinformatically search for similar coding-sequence hairpins throughout all bacteria, leading to the discovery of many stable RNA structures with conserved locations throughout very divergent bacteria (e.g. Streptomyces, Escherichia coli, Bacillus subtilis). |
URI: | http://hdl.handle.net/11375/22027 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Moody_Matthew_J_201709_PhD.pdf | 11.08 MB | Adobe PDF | View/Open |
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