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http://hdl.handle.net/11375/21666
Title: | Studying the Effects of p120 and Kaiso-Mediated Gene Regulation on Epithelial-to-Mesenchymal-Transition |
Authors: | Almardini, Mai |
Advisor: | Daniel, Juliet |
Department: | Biology |
Keywords: | Kaiso-mediated, gene regulation, epithelial-to-mesenchymal-transition, E-cadherin, cells |
Publication Date: | Nov-2007 |
Abstract: | <p> Downregulation of E-cadherin is a frequent event in epithelial cancers and it correlates with weakened cell-cell adhesion and the induction of an epithelial-to-mesenchymal transition (EMT). It is postulated that E-cadherin downregulation liberates the catenin p120 and allows p120's translocation to the nucleus where it interacts with and functionally regulates the novel BTB/POZ transcription factor, Kaiso. Kaiso mediates transcriptional repression of various tumourigenesis-associated genes via methylated CpG dinucleotides or a sequence-specific Kaiso binding site (KBS). The Kaiso/p120 interaction has been detected in E-cadherin expressing cells of various origins, but is seldom detected in N-cadherin expressing cells or cells that have undergone EMT. We hypothesize that p120 and Kaiso play a role in EMT by modulating the expression of EMT-associated genes. We demonstrated that TGF-β-induced EMT occurs in a dose- and time-dependent manner in NMuMG cells but not in FHL-124 cells. In both cells lines, the Kaiso/p120 interaction occurred irrelevant of EMT induction by TGF-β. In NMuMG cells, the expression of p120 increased with EMT induction, while the expression of Kaiso remained unchanged. Finally, misexpression of Kaiso and p120 in mammary epithelial cells affected TGF-β-mediated EMT induction by delaying the upregulation of the positive mesenchymal markers, N-cadherin and α-SMA.</p> |
URI: | http://hdl.handle.net/11375/21666 |
Appears in Collections: | Digitized Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Almardini_Mai_2007Nov_Masters..pdf | 8.53 MB | Adobe PDF | View/Open |
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