The Synthesis of RNA by Isolated Rat Liver Mitochondria

Fukamachi, Seijiro

Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/19440

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DC Field	Value	Language
dc.contributor.advisor	Freeman, Karl B.	-
dc.contributor.author	Fukamachi, Seijiro	-
dc.date.accessioned	2016-06-02T16:27:05Z	-
dc.date.available	2016-06-02T16:27:05Z	-
dc.date.issued	1972-02	-
dc.identifier.uri	http://hdl.handle.net/11375/19440	-
dc.description.abstract	<P> Mitochondria contain DNA which is distinct from nuclear DNA. The capacity of isolated rat liver mitochondria to synthesize RNA and the types of RNA synthesized were examined in order to determine the genetic function of mitochondrial DNA. </p> <p> It was demonstrated that isolated rat liver mitochondria synthesize RNA, incorporating [3H]UTP, [3H] ATP,[3H]CTP and [3H]GTP in a DNA-dependent reaction. In addition, the DNA-independent incorporation of [3H]CTP and [3H]ATP suggested metabolic turnover of the CCA end of mitochondrial tRNA. </p> <P> Analysis of the , newly-synthesized RNA by sucrose density gradient centrifugation and agarose-polyacrylamide gel electrophoresis demonstrated that mitochondrial ribosomal RNA was synthesized in a DNA-dependent process. It is concluded that one of the genetic functions of mitochondrial DNA is to code for mitochondrial ribosomal RNA. </p>	en_US
dc.language.iso	en	en_US
dc.subject	Synthesis of RNA	en_US
dc.subject	Liver Mitochondria	en_US
dc.subject	rat	en_US
dc.subject	nuclear DNA	en_US
dc.title	The Synthesis of RNA by Isolated Rat Liver Mitochondria	en_US
dc.contributor.department	Biochemistry and Biomedical Sciences	en_US
dc.description.degreetype	Thesis	en_US
dc.description.degree	Doctor of Philosophy (PhD)	en_US
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